Coding

Part:BBa_K3221204

Designed by: Yaxin Luo   Group: iGEM19_SCU-China   (2019-10-04)


Reporter sequence of delay system

BBa_K3221204 is consisted of pMET3(BBa_K3221201), GAL4(BBa_K3221208) and pGAL1-yeGFP(BBa_K3221200). In our project, MET3 promoter and GAL4-pGAL1 construct the delay system to ensure the adequate cultivation of yeast before the expression of cns1-cns2 fusion protein and yeGFP is used as the reporter. And the delay efficiency is adjustable and can be controlled by the presence of different concentrations of methionine in the medium. This system can be also used for other delay systems in Saccharomyces cerevisiae.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 302
    Illegal XhoI site found at 718
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3560
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 399
    Illegal BsaI site found at 637
    Illegal BsaI.rc site found at 2313
    Illegal BsaI.rc site found at 4609
    Illegal SapI site found at 1002


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Categories
Parameters
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